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OBJECTIVE: To analysis the dynamic change of cytokines in patients with occupational trichloroethylene-induced medicamentosa-like dermatitis(OMDT) at the initial stage of treatment. METHODS: Twenty-two cases of early onset OMDT with no glucocorticoid treatment history were selected as the research subjects by judgment sampling method. Blood samples were collected on the 1 st, 2 nd, 3 rd, 4 th and 5 th weeks after admission and on the day of hospital discharge. The levels of tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ), interleukin(IL)-5, IL-6 and IL-10 in plasma samples were measured by the enzyme linked immunosorbent assay. RESULTS: The five cytokines in patients with exfoliative dermatitis showed an increasing trend at the initial stage of treatment. Among them, the levels of TNF-α, IL-5 and IL-10 reached a peak and then dropped rapidly to form a plateau, and the levels of IFN-γ and IL-6 were slightly increased and the duration of increase was shorter than that of other cytokines. The levels of TNF-α, IFN-γ, IL-5 and IL-6 in patients with erythema multiforme remained within the detection limits in the detection process. Only a few patients showed a short-term increase, the IL-10 level showed a slight increase at the initial stage and then decreased to the plateau stage. The levels of TNF-α, IFN-γ and IL-6 in patients with bullous epidermal necrolysis increased rapidly at the initial detection stage for a short period of time, and then decreased sharply. The level of IL-5 remained at the detection limit, and the IL-10 level showed alternative rising and falling pattern. Part of the dynamic change of cytokines in patients with exfoliative dermatitis and bullous epidermal necrolysis was similar. CONCLUSION: The levels of TNF-α, IFN-γ, IL-5, IL-6, and IL-10 in OMDT patients changed with the progression of the disease at the early treatment stage, and the degree of change was related to the type of rash. Among them, the levels of TNF-α and IL-10 showed dynamic changes due to the progression of the disease, which could be considered as effect biomarkers to evaluate the severity and progression of the disease, and provide a reference for the rational treatment of patients.
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OBJECTIVE: To obtain information on the toxicity of lufenuron on the reproduction ability and the growth and development of offspring in female and male rats through two-generation reproduction toxicity study. METHODS: The specific pathogen free healthy SD rats were randomly divided into control group and low-, medium-and high-dose lufenuron groups, with 60 rats in each group, half females and half males. Rats in the low-, medium-and high-dose lufenuron groups were respectively fed with lufenuron at the dose of 5.0, 20.0 and 80.0 mg/(kg body weight·day) for 8 weeks before mating. The control group was fed with standard foot. The reproductive index, brain and reproductive organ coefficients and pathological changes were observed in P and F1 parents. The birth and growth indexes of the offspring were measured. RESULTS: i) P generation: from the 14 th day, the female rats in the medium-dose group had lower body weight than that of the female control group(P<0.05); from the 35 th day, the body weight was lower than that of the female low-dose group(P<0.05). From the 14 th day, the female rats in the high-dose group had lower body weight than that of the other three female groups(P<0.05). From the 14 th day, the male rats in the medium-and high-dose groups had lower body weight than that of the male control group and low-dose group(P<0.05). The body weight of pregnant rats in the parental high-dose group was lower than that of the control group, low-dose group, and medium-dose group at day 0, 7, 14, 19 of the pregnancy duration(P<0.05). The body weight of pregnant rats in the parental medium-dose group was lower than that of the low-dose group on day 0 of the pregnancy duration, and lower than that of the control and low-dose groups on day 7 and 14(P<0.05). The conception rate, the new-borne survival rates and the feeding survival rate of female rats in the high-dose group was lower than that of the other three female groups(P<0.008). The new-borne feeding survival rate of female rats in the medium-dose group was lower than that of the control group and low-dose group(P<0.008). The organ coefficients of brain in female rats in the medium-and high-dose groups were higher than that of the female control group and low-dose group(P<0.05). The organ coefficients of brain and testis in male rats in the medium-and high-dose groups were higher than that of the control group and low-dose group(P<0.05). The organ coefficient of epididymis in male rats in the high dose group was lower than that of the other three male groups(P<0.05). ii) F1 generation: the body weight of female rats in the low-and medium-dose group was higher than that of the female control group on the 42 th day(P<0.05). The body weight of male rats in the low-dose group was higher than that of the male control group on the 42 th, 49 th, and 56 th days(P<0.05). The body weight of male rats in the medium-dose group was higher than that of the male control group on the 14 th, 21 th, 42 th, 49 th, and 56 th days(P<0.05). The new-borne survival rate in the low-dose group was lower than that of the control group(P<0.017). The body weight of new-borne rats in the high-dose group on day 4 of birth was lower than that in the other three female groups(P<0.05). iii) F2 generation: the body weight of male rats in the male medium-dose group was lower than that in the control group on day 21 of birth(P<0.05). CONCLUSION: The reproductive and developmental toxicity of lufenuron is found in rats in the medium-and high-dose groups. Toxicities including low body weight, conception rate, new-borne survival rate and feeding survival rate are found in P generation; low body weight and feeding survival rate are found in F1 generation; low body weight is found in male F2 generation. The no-observed-adverse-effects levels of lufenuron in two-generation reproductive study are 5.87 mg/(kg·d) for females and 5.09 mg/(kg·d) for males in SD rats.
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OBJECTIVE: To observe the pathological changes of rat silicosis model at different time points. METHODS: The specific pathogen free SD rats were randomly divided into control group and 7, 15, 21, 30, 45, 60, 75 and 90 day model groups based on their body weight, with 5 rats in each group. Non-exposed endotracheal intubation was performed. Silicosis rat model was established by intratracheal instillation of 250 g/L silica suspension in each rat, and 0.9% sodium chloride solution was perfused into the trachea of rats in the control group. The rats in the control group were sacrificed on the 90 th day after exposure, and the model rats in the other 8 groups were sacrificed on the 7 th, 15 th, 21 st, 30 th, 45 th, 60 th, 75 th and 90 th days after the end of exposure. The gross appearance of the lung tissue of rats was observed. The rat lung tissues were stained with hematoxylin-eosin and Masson staining to observe the pathological changes, and Ashcroft score was evaluated. RESULTS: The gross observation showed that the lungs of rats in the model groups had varying degree of gray changes, hardened texture, and spots and nodules on the surface of the lobes. These changes were aggravated with the increase of time after dust exposure. The results of histopathological examination of the lungs showed that the rats developed acute alveolar inflammation, and a large number of macrophages and neutrophils were seen in the lung tissues in the 7 th and 15 th day model groups. Cellular nodules appeared in the lung tissue, and fibrosis appeared in the center of the nodule in the rats of 21 st, 30 th, and the 45 th day model groups; the silicosis nodules appeared in the lung tissues of rats in the 60 th, 75 th, and 90 th day model groups, and the small nodules gradually merged into larger ones. Simultaneously, with the increase of time after dust exposure, the lung tissue of rats gradually showed severe pulmonary fibrosis. The lung organ coefficient and Aschcroft score of rats increased with the increase of time after dust exposure(P<0.01). CONCLUSION: The rat lung changes after dust exposure. Acute alveolar inflammation occurs on the 7 th to 15 th day after dust exposure; cellular nodules develop on the 21 st to 45 th day after dust exposure; silicosis nodules develop on the 60 th to 90 th day after dust exposure. The severity of lung fibrosis after dust exposure showed a time-effect relationship in rats.
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OBJECTIVE: To investigate the correlation between plasma cytokine levels and liver functions in patients with occupational medicamentosa-like dermatitis due to trichloroethylene(OMDT). METHODS: A total of 22 OMDT patients were selected as research subjects using judgment sampling method. Blood samples were collected from patients on the 1 st, 2 nd, 3 rd, 4 th, and 5 th week of admission and the day of hospital discharge. The automatic biochemical instrument was used for detecting the index of serum liver function. The levels of cytokines including tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ), interleukin(IL)-5, IL-6, and IL-10 in plasma were measured by enzyme-linked immunosorbent assay. The Spearman correlation analysis was performed to analyze the correlation between cytokines and liver function in 15 patients with exfoliative dermatitis. RESULTS: The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), direct bilirubin(DBIL), glutamyl transpeptidase(GGT), and total bile acid(TBA) of OMDT patients on the 1 st week of admission increased(P<0.05), while total protein(TP) and albumin(ALB) decreased(P<0.05) compared with the results at discharge(a stage of recovery). The correlation analysis results of patients with exfoliative dermatitis showed that: the levels of TNF-α, IFN-γ and IL-6 were negatively correlated with the levels of TP and ALB respectively(P<0.05), the level of IL-5 was negatively correlated with TBIL(P<0.05), and the level of IL-10 was negatively correlated with ALB(P<0.05) in the 1 st week. The level of IL-6 was positively correlated with ALT(P<0.05) in the 2 nd week. The level of TNF-α was positively correlated with TBIL(P<0.05), the level of IL-10 was positively correlated with AST(P<0.05) in the 3 rd week. The levels of TNF-α and IL-10 were positively correlated with AST and ALT respectively(P<0.05), the level of IFN-γ was positively correlated with AST(P<0.05) in the 4 th week. The levels of IL-6 and IL-10 were positively correlated with ALT and GGT(P<0.05), and the levels of TNF-α and IFN-γ were positively correlated with AST(P<0.05) in the 5 th week. The level of TNF-α was negatively correlated with DBIL(P<0.05) and was positively correlated with TBA(P<0.05) at discharge.CONCLUSION:s Patients with OMDT are frequently accompanied with severe liver function damage at the early stage. The level of plasma cytokines(TNF-α, IFN-γ, IL-6 and IL-10) might correlate with the severity of liver dysfunction.
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OBJECTIVE: To investigate the chronic toxicity and carcinogenicity of kresoxim-methyl in rats. METHODS: Specific pathogen free SD rats were randomly divided into control group and low-, medium-and high-dose groups according to the body weight of rats, 120 rats in each group with half male and half female rats. The chronic toxicity and carcinogenesis was induced in rats for 104 weeks by oral feeding. The dose of kresoxim-methyl in feed of male and female rats was 0, 75, 300 and 1 200 mg/kg. During the process of experiment, the body weight of rats was weighed. The blood biochemistry, organ coefficient and histopathology were examined at the end of the exposure, and the tumor incidence was calculated. RESULTS: There was no significant difference in mortality of the female or male rats in the four groups(P>0.05). At the 32 nd, 48 th and 56 th week after exposure, the body mass of female rats in the high dose group was lower than that in control group(P<0.05); at the 8 th, 16 th, 24 th and 32 nd week, the body mass of male rats in the high dose group was lower than that in the control group(P<0.05). The organ coefficients of heart and adrenal gland of female rats in the high dose group were higher than those in the control group and the low dose group(P<0.05). The organ coefficient of liver of male rats in the high dose group was lower than that in the control group(P<0.05). The alkaline phosphatase of male rats in the three dose groups was lower than that in the control group(P<0.05). The blood glucose of male rats in the high dose group was higher than that in the control group(P<0.05). The aspartate aminotransferase of male rats in the high dose group was lower than that in the control group(P<0.05). There was no significant difference among the three indexes in female rats(P>0.05). The tumor incidence of the control group and the low, medium and high dose groups were 68.3%, 75.0%, 75.0% and 78.8%, respectively, with no significant difference(P>0.05). The tumor incidence of the female rats was higher than that of the male rats(87.0% vs 61.5%,P<0.01).The tumor multiplicity of the above four groups were 38.3%, 35.8%, 35.0%, 39.8%, respectively, with no significant difference(P>0.05). The tumor multiplicity in female rats was higher than that in male rats(56.9% vs 17.6%,P<0.01). CONCLUSION: The no observed adverse effect level of kresoxim-methyl to female and male SD rats was 24.726 and 20.002 mg/(kg·d), respectively. No carcinogenicity of kresoxim-methyl to SD rats was observed.
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OBJECTIVE: To evaluate the diagnostic ability of toxicity pathology in patho-toxicological testing institutions in China. METHODS: The institutions participated in the 2018 Interlaboratory Comparison Activity of Toxicity Pathology Testing(hereinafter referred to as reference unit) were selected as the research subjects. The heart, spleen, skin, soft tissue, liver and mammary gland of SD rats of different groups in the 2-year carcinogenesis test were selected. The femur, knee joint and nose of Beagle dogs in the 4-week toxicity test and a total of 10 pathological tissues were selected as the comparison samples. The pathological diagnosis was carried out by the pathological diagnostic personnel of the reference unit, and the diagnostic results were reported. The expert appointed by the Toxicology and Pathology Committee of Chinese Toxicology Association compared the diagnostic results with the appointed value. RESULTS: A total of 167 pathological diagnostic personnel from 75 reference units in 24 provinces and municipalities participated in the comparison activity. The reference units were mainly distributed in East China, South China and North China, accounting for 77.3%(58/75). Totally 75 reference units fed back 750 effective diagnostic results. The qualified rates of diagnosis on heart, spleen, skin, soft tissue and breast samples were higher than 60.0%. The qualified rates of diagnosis on femur and knee joint, and nose samples were low(30.7% and 6.7%, respectively). There were 1(1.3%), 46(61.4%) and 28(37.3%) reference units rated as unqualified, qualified and excellent, respectively. CONCLUSION: Most of the testing institutions in China have a high level of patho-toxicological diagnostic ability, that can provide reliable diagnostic results for toxicology safety evaluation tests.
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OBJECTIVE: To explore the relationship between serum mannose-binding lectin( MBL) and T helper cell 17( Th17)/regulatory T cells( Treg) balance in patients with silicosis. METHODS: A total of 101 male patients with silicosis were selected in silicosis group and 62 health individuals in control group using the cross-sectional study. The level of serum MBL was measured by enzyme linked immunosorbent assay. The ratio of Th17/Treg was recorded by flow cytometry.The relative expression of retinoid-related orphan nuclear receptor γt( RORγt) and forkhead box 3( Foxp3) mRNA in peripheral blood mononuclear cell were tested by real-time polymerase chain reaction method. RESULTS: The level of serum MBL in silicosis group was higher than that of control group( P < 0. 01). The ratio of Th17 cells and the relative expression of RORγt mRNA increased in silicosis group( P < 0. 05),while the ratio of Treg cells and the relative expression of Foxp3 mRNA decreased in silicosis group( P < 0. 05) compared to the control group. The level of serum MBL had negative correlation with forced expiratory volume in the first second,forced vital capacity and forced expiratory flow( P < 0. 05) in patients with stage Ⅰ and Ⅱ silicosis. Meanwhile,the level of serum MBL had negative correlation with Th17 ratio and RORγt mRNA relative expression( P < 0. 05),and positive correlation with Treg ratio and Foxp3 mRNA relative expression( P < 0. 05). CONCLUSION: MBL might participate in the development of silicosis through regulating the balance of Th17/Treg cells.
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OBJECTIVE: To investigate the effects of 1,2-dichloroethane(1,2-DCE) subacute exposure on depression in rats as well as the relevant mechanism of monoamine neurotransmitters. METHODS: The specific pathogen free male SD rats were randomly divided into control group, low-, medium-, and high-dose groups, with 10 rats in each group. The rats in these 4 groups were intra-gastrically administered with 1,2-DCE(diluted in corn oil) at the dose of 0, 20, 40, 80 mg/kg body weight, every other day for 14 times. After exposure, the behavior change of rats was observed by open-field test, sucrose preference test and forced swim test. The levels of the monoamine neurotransmitters including 5-hydroxytryptamine(5-HT), noradrenaline(NA) and dopamine(DA) in prefrontal cortex, hippocampus, and striatum of rats were analyzed by high performance liquid chromatography-electrochemical detection method. RESULTS: The number of rearing, time and distance of central area, sucrose preference index of mice in medium and high dose groups were decreased(P<0.05), while immobility time of forced swim test was increased(P<0.05) when compared with the mice in control group. The levels of 5-HT, NA and DA in prefrontal cortex, hippocampus, and striatum decreased with the increase of 1,2-DCE exposure(P<0.05), showing a dose-effect relationship. The levels of 5-HT, NA and DA in prefrontal cortex, hippocampus, and striatum in the high-dose group were lower than that of control group(P<0.05). CONCLUSION: The subacute exposure of 1,2-DCE can induce depression-like behavior in rats. The mechanism might be related to the reduction of monoamine neurotransmitters in striatum, hippocampus and prefrontal cortex.
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OBJECTIVE: To explore the effects of sub-acute inhalation of 1-bromopropane( 1-BP) on the ultrastructure of cerebral cortex,hippocampus,cerebellum,and brainstem in male rats. METHODS: Specific pathogen free healthy male Wistar rats were randomly divided into control group and exposure group with 6 rats in each group. The rats of exposure group received 1-BP vapor at a concentration of 5 000 mg/m3. The rats in the control group were given fresh air in a dynamic inhalation chamber system for 4 weeks(6 hours/day,5 days/week). After the end of the exposure,the cerebral cortex,hippocampus,cerebellum and brainstem of rats were collected and the ultrastructural changes were observed under transmission electron microscope( TEM). RESULTS: After 3 weeks of exposure to 1-BP,the rats in the exposure group began to have unresponsiveness and decreased muscle strength in hind limbs. The body weight of exposure group was lower than that of control group from the 1 st to the 4 th week( P < 0. 05). TEM results showed destroyed structure of the myelin sheath in the region of cerebral cortex, hippocampus, cerebellum and brainstem, and irregular nucleus, vacuolar degeneration,increased lysosome of endoplasmic reticulum,mitochondrion swelling of neuron cells,karyopyknosis of astrocytes and vacuolation in the neurite of astrocytes located in the blood brain barrier( BBB). CONCLUSION: 1-BP sub-acute inhalation exposure could damage the myelin,neuron,astrocyte and BBB in male rats. The demyelination of nerve fiber and decreased permeability of BBB was particularly noticeable.
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OBJECTIVE: To understand the influence of shift work on common risk factors of cardiovascular disease in male workers in petrochemical enterprises.METHODS: A total of 981 male workers in a petrochemical enterprise were selected as study subjects by judgment sampling method.According to the current status of work shift,619 workers were in the shift group and 362 in the non-shift group.The differences in the related indicators of common risk factors of cardiovascular disease in these two groups were compared.RESULTS: The systolic blood pressure,diastolic blood pressure,uric acid,fasting blood glucose( FBG),and total cholesterol( TC) levels in workers of the shift group were higher than that in the non-shift group( P < 0.01).The diastolic blood pressure,serum FBG,TC,triglyceride levels,and obesity detection rate in the subgroup workers with a shift length ≥ 5.0 years were higher than that in the subgroup with shift length < 5.0 years( P < 0.05).The prevalence of hypertension,hyperglycemia,hyperlipidemia,and hyperuricemia in the study population were 9.3%,1.4%,19.9% and 33.4%,respectively.The prevalence of hyperuricemia increased with the increased working shift length( P < 0.01).The multivariate logistic regression analysis results showed that,the length of shift work was a risk factor for hyperuricemia( P < 0.01) after excluding the confounding factors such as age,body mass index,smoking and alcohol consumption.With the increase of the length of shift work,the risk of hyperuricemia increased.There was no correlation between shift working length and hypertension,hyperglycemia,and hyperlipidemia( P > 0.05).CONCLUSION: Shift work can increase the risk of hyperuricemia in male workers; shift work was not found to increase the risk of hypertension,hyperglycemia,and hyperlipidemia.
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OBJECTIVE: To investigate the therapeutic effect of electroacupuncture on peripheral nerve damage induced by 1-bromopropane( 1-BP) exposure.METHODS: A total of 25 specific pathogen free healthy adult male Wistar rats were randomly divided into blank control group( n = 5),model control group( n = 10),and electroacupuncture treatment( EA) group( n = 10).Rats in the blank control group were not exposed to 1-BP and treated with electroacupuncture.The rats in model control group and EA group were placed in a dynamic inhalation exposure cabinet with 1-BP at concentration of 5 000 mg/m~3.The rats were continuously exposed to 1-BP 8 hours per day,5 days a week,for 4 weeks.At the 3 rd day after the end of the exposure,the EA group was treated with electroacupuncture on“Zu sanli”and“Huantiao”points for 4 courses.Each course included 20 minutes each time,once per day for 7 consecutive days.The body weight,the motor nerve conduction velocity( MCV) and sense nerve conduction velocity( SCV) of sciatic nerves on both posterior limbs of the rats were measured.RESULTS: During the course of 1-BP exposure,the rats in the EA and model control group showed reduction of eating,drinking and activities,limited autonomic activities and their hind limbs dragged.The MCV and SCV of posterior limb sciatic nerve of rats in the model control group were slower than that of the control group at the 4 th,6th and 8th week and the 0 week of the same group( P < 0.05).The MCV and SCV of posterior limb sciatic nerve of rats in the EA group improved with the increase of treatment time( P < 0.05),and those at the 6th and 8th weeks of the experiment( corresponding to the 2nd and 4th week after treatment) were faster than that of the model control group at the same time( P < 0.05).The SCV of the posterior limb sciatic nerve in the EA group recovered to normal level 4 weeks after treatment compared with the blank control group( P < 0.05).CONCLUSION: Electroacupuncture treatment can promote the recovery of peripheral nerve damage in rats with 1-BP poisoning.
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OBJECTIVE: To assess the effect of shift work on hypertension in petrochemical production workers. METHODS: Totally 2573 workers were recruited from a petrochemical company by convenience sampling method. We collected the basic information of participants via questionnaire and made occupational physical examination in these subjects to evaluate the relationship shift work with hypertension. RESULTS: The results showed that the prevalence of hypertension in the present study was 15. 1%,and the shift workers were with significant higher prevalence compared with the non-shift workers( 15. 9% vs 10. 7%,P < 0. 05). The systolic blood pressure levels were significant higher in shift work group than that in non-shift work group [( 119. 8 ± 14. 9) vs( 116. 6 ± 13. 7) mmHg,P < 0. 01]. After adjusted for age,gender,education,body mass index,family history of hypertension,and other confounding factors,multiple logistic regression analysis showed that the risk of hypertension in shift work group was 1. 49 times( 95% confidence interval was 1. 05-2. 12)than that of non-shift work group( P < 0. 05). By using stratified analysis by the duration of shift work in the shift workers,the risk of hypertension in the duration of 10-year and 20-year groups were higher than that of the duration less than 10-year group( P < 0. 05). CONCLUSION: Shift work exposure increases the prevalence of hypertension by affecting systolic blood pressure,and this risk can be enhanced with increasing duration of shift work.
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OBJECTIVE: To observe the effects of bone marrow mesenchymal stem cells( BMSCs) on the treatment of pulmonary fibrosis in silicosis mice. METHODS: Specific pathogen free healthy male C57BL/6 mice were randomly divided into control group,silicosis group and treatment group with 10 mice in each group. The mice of the control group were given one intra-tracheal injection of 20. 0 μL 0. 90% sodium chloride solution. The silicosis group and treatment group received one 20. 0 μL( mass concentration 250 g/L) of silica dust suspension. After 4 weeks,mice in treatment group were injected with 250. 0 μL of BMSCs suspension( cell density 2 × 10~9/L) by tail vein and silicosis group injected with 250. 0 μL of 0. 90% sodium chloride solution instead,once a week with continuous treatment for 4 weeks. Control group was not given any treatment. Mice were euthanized two weeks after the last treatment. Pathological sections were observed,pulmonary fibrosis score( Ashcroft scores) was marked. Lung coefficient was measured. Lung tissue hydroxyproline( HYP) level and serum transforming growth factor β1( TGF-β1) level were measured. The level of pulmonary fibrosis was scored and the percentages of T helper cell 17( Th17 cell) and regulatory T cell( Treg cell) of spleen and hilar lymph node( HLN) were measured by flow cytometry. RESULTS: The results of lung histopathological examination showed that the pulmonary fibrosis was severe in silicosis group. Massive collagen fiber accumulation and silicotic nodule were found. In treatment group,fibrosis was mild,little collagen fiber accumulation and silicotic nodule were found. The lung coefficient,Aschcroft scores,lung tissue HYP level,serum TGF-β level and the percentage of Th17 cell of spleen and HLN in silicosis group were higher than that of control group( P < 0. 05),while the above indexes of treatment group were lower than that of silicosis group( P < 0. 01). The percentage of Treg cell of spleen and HLN in silicosis group were lower than that of control group( P < 0. 05),while those indexes of treatment group were higher than that of silicosis group( P < 0. 01).CONCLUSION: BMSCs could effectively alleviate the pulmonary fibrosis in silicosis mice and correct the imbalance of Th17/Treg.
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OBJECTIVE: To explore the repair effects of bone marrow mesenchymal stem cells( BMSCs) on hematopoietic injury induced by benzene poisoning in mice. METHODS: Five specific pathogen free healthy male Kunming mice were selected to obtain BMSCs through bone marrow attachment culturing method. The Kunming mice were randomly divided into poisoning group and BMSCs transplantation group,18 mice in each group,after the benzene poisoning model was established by subcutaneous multi-point injection of benzene and oil mixture 3 times/week,10 weeks continuously. Each group was injected through tail vein with 250. 0 μL 0. 9% sodium chloride solution or 250. 0 μL BMSCs suspension( cell density 2 × 109/L) once per week for 4 weeks,respectively. The control group( 10 mice) was not given any treatment.Mice were euthanized 2 weeks after treatment. The blood routine examination was conducted. Nucleated cells in bone marrow were observed after Giemsa staining. The clones of hemopoietic progenitor cells were counted and the levels of serum interferon-γ( IFN-γ) were examined using enzyme-linked immune sorbent assay. RESULTS: The mouse model of chronic benzene poisoning was established successfully. After the BMSCs transplantation treatment,the white blood cell count,platelet count,red blood cell count,hemoglobin level and bone marrow nucleated cell as well as granulocyte-macrophage colony forming unit( CFU-GM) in benzene poisoning group were significantly decreased compared with control group( P <0. 01),while those indexes of BMSCs treatment group were higher than that of benzene poisoning group( P < 0. 05). The counts of platelet,red blood cell,bone marrow nucleated cell and CFU-GM in BMSCs treatment group were significantly lower than that of control group( P < 0. 05). The level of serum IFN-γ in benzene poisoning group was higher than that of control group( P < 0. 01),and serum IFN-γ level in BMSCs treatment group was lower than that of benzene poisoning group( P < 0. 01). There was no significant difference of IFN-γ level in BMSCs treatment group compared with control group( P > 0. 05). CONCLUSION: BMSCs have repair effects on hematopoietic system injury caused by benzene poisoning.
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OBJECTIVE: To explore the effect of 1,2-dichloroethane(1,2-DCE) induced apoptosis on the expression of related proteins in human neuroblastoma cells(SH-SY5 Y cells). METHODS: SH-SY5 Y cells were cultured in complete medium with 1,2-DCE at final concentrations of 0,10,20,30,40,50,60,70 and 80 mmol/L. After being cultured for24 hours,the apoptosis of SH-SY5 Y cells was tested by flow cytometry using annexin Ⅴ-fluorescein isothiocyanate and propidium iodide. Western blot was used to detect the protein expression of P53,B cell lymphoma/leukmia-2(BCL-2)and BCL-2 associated X protein(BAX). RESULTS: At 1,2-DCE concentrations of 0-80 mmol/L,the total apoptosis rate of SH-SY5 Y cells increased with 1,2-DCE concentrations in a dose-dependent manner(P < 0. 01). At 1,2-DCE concentrations of 30-80 mmol/L,the early apoptosis rate and total apoptosis rate of SH-SY5 Y cells increased significantly than the control group(P < 0. 05). Compared with the other groups,the protein expression of P53 was the lowest when the1,2-DCE concentration was 20 mmol/L(P < 0. 05),and the protein expression of BCL-2 and the BCL-2/BAX ratio were the lowest when the 1,2-DCE concentration was 70 mmol/L(P < 0. 05). There is no dose-response relationship in the1,2-DCE concentrations and the protein expression levels of P53,BCL-2 and BAX,and BCL-2/BAX ratio. Linear multiple regression analysis revealed that the total apoptosis rate of SH-SY5 Y cells treated with 1,2-DCE was associated with the protein expression of P53 and BCL-2,and BCL-2/BAX ratio(P < 0. 05). CONCLUSION: 1,2-DCE could inhibit the apoptosis of SH-SY5 Y cells. The mechanisms may be related to the changes of P53 and BCL-2 protein expression,and BCL-2/BAX relative amount.
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OBJECTIVE: To explore the immune cytotoxicity effect and its mechanism of trichloroethylene( TCE) on activated human T cells. METHODS: a) Different concentrations of TCE( 0. 32,0. 63,1. 25,2. 50,5. 00,10. 00 mmol / L)were used to treat activated T cells [activated with cluster of differentiation( CD) 3 and CD28] respectively. Dimethyl sulfoxide( DMSO) was used in the solvent group and the control group used no TCE or DMSO. The survival rate of activated T cells was calculated using CCK-8 assay after being cultured for 24 hours. b) Different concentrations of TCE( 0. 00,2. 50,5. 00 mmol/L) were used to treat activated T cells. The apoptosis of cells was detected using flow cytometry. c) Different concentrations of TCE( 0. 00,0. 32,0. 63,1. 25,2. 50,5. 00 mmol / L) were used to treat activated T cells and the level of cytokines as interleukin( IL)-2 and IL-6 in cell culture supernatant was detected using enzyme linked immunosorbent assay after culturing for 24 hours. d) The control group and TCE treatment group of activated T cells were treated with 0. 00 and 5. 00 mmol / L TCE respectively. Cells were collected after culturing 0,30,60 and 120 minutes. Western Blot was used to detect the protein expression of signal transducers and activators of transcription3( STAT3) and phospho-STAT3( p-STAT3). RESULTS: a) After 24-hour-exposure to TCE,the activated T cell survival rate of 10. 00 mmol / L TCE treatment group were significantly lower than that in the control group and DMSO group( P <0. 05). b) There were no significant differences in cell apoptosis of activated T cells after treatment with 0. 00,2. 50 and5. 00 mmol / L TCE( P > 0. 05). c) In groups treated with different concentrations of TCE( 0. 32,0. 63,1. 25,2. 50,5. 00 mmol / L),the level of IL-2 and IL-6 in the cell culture supernatant of activated T cells were significantly higher than that in the control group( P < 0. 05). With the increasing of TCE exposure doses,the levels of IL-2 and IL-6 significantly increased( P < 0. 01) with dose-effect relationship. Compared with the control group,the levels of IL-17 A,interferongamma and transforming growth factor-beta in cell culture supernatant of activated T cells of the TCE treatment groups were no significant differences( P > 0. 05). d) The expression of p-STAT3 protein was low in the control group at different times. The expression of p-STAT3 protein in TCE treatment group was low at 0 minute,but increased at 30,60,120 minutes. The expression of p-STAT3 protein in TCE treatment group was higher than that in the control group at different time points. The levels of STAT3 total protein in TCE treatment group and the control group were similar at different time points,and were higher than the p-STAT3 proteins. CONCLUSION: TCE at 5. 00 mmol / L had no observed toxic effect on activated T cells. High doses of TCE( ≥10. 00 mmol / L) showed cytotoxic damages to activated T cells,and low doses of TCE( ≤5. 00 mmol / L) could stimulate activated T cells to secrete IL-2 and IL-6. Treatment of TCE at 5. 00 mmol / L on activated T cells could up-regulated the level of p-STAT3.
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Objective To investigate the short-term effect of pelvic floor muscle training (PFM) with biofeedback on stress urinary incontinence (SUI) in postpartum and post-menopausal women.Methods According to the different period that the SUI occurs,107 women with SUI were divided into two groups:the group of SUI in postpartum with 60 women,and the group of SUI in post-menopausal with 47 women.PFM with biofeedback was performed on all patients for 8 weeks.One hour pad-weighing test,voiding diary,transperineal three-dimensional ultrasound and female pelvic floor muscle assessment were recorded before and after treatment.Results There was statistically significant difference in 1 hour padweighing test between pre-treatment and post-treatment for the group of SUI in postpartum (the negative,mild,moderate,and severe cases of post-treatment:21,24,14,1,of pre-treatment:0,30,28,2; P＜0.05),and the group of SUI in post-menopausal (the negative,mild,moderate,and severe cases of post-treatment:7,22,11,7,of pre-treatment:0,14,25,8; P＜0.05).The strength of the pelvic floor muscles of type Ⅰ and type Ⅱin two groups after treatment were significantly different from those in pre-treatment (P＜0.01).The efficient rate of improvement in symptoms after treatment in the group of SUI in postpartum was 88％ (53/60) and the cure rate was 38％ (23/60).While the efficient rate in the group of SUI in post-menopausal women was 64％(30/47) and the cure rate was 15％ (7/47).There was statistically significant difference in the development of symptoms in two groups after treatment (P=0.003).Conclusion PFM with biofeedback is an effective treatment for SUI in postpartum and post-menopausal women,especially for postpartum ones.
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Glioma is the most frequently observed primary tumor of the central nervous system in adults. Among the glioma cases, more than three quarters of patients suffer from high-grade gliomas. High-grade glioma is not only a high-degree malignant tumor but is also an easily recurring disease after surgery with a very poor prognosis. Radiotherapy plus concomitant chemotherapy after operation is the standard treatment strategy for high-grade gliomas, which could increase the survival rate of patients. However, the curative effect is really not satisfactory because it could only guarantee a limited survival time. Over the recent years, molecular-targeted treatment has increasingly drawn the attention of scholars with the continuous development in glioma treatment, thereby becoming the hotspot among researchers. Vascular endothelial growth factor (VEGF) is highly expressed in glioma and in the tissues surrounding the cancer cells. VEGF could regulate tumor growth by inducing endothelial cell proliferation, growth, migration, and by increasing the vascular permeability. Hence, VEGF becomes an effective target for the treatment of glioma. Bevacizumab is a monoclonal antibody that can specifically prevent the combination of VEGF and its receptor, thereby inhibiting the formation of tumor blood vessels. At the same time, bevacizumab can normalize the tumor blood vessels, improve the permeability of blood vessels, and increase the effectiveness of drug concentration in the tumor tissues, thereby achieving anticancer efficacy. In this paper, the mechanism of bevacizumab is introduced. The research progress in the application of bevacizumab alone, as well as in combination with chemotherapy or other drugs, for the high-grade glioma treatment will be summarized.
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AIM: To observe the effects of ketamine on preventing the increase of postoperative morphine requirement induced by fentanyl.METHODS: Sixty women undergoing total abdominal hysterectomy by spinal anaesthesia were assigned to 4 groups consisting of placebo(normal saline,C),fentanyl(3 bolus of 1 ?g/kg,at 15 min intervals,F),ketamine(infusion of 15 ?g?kg-1?min-1 ketamine from the skin incision until 20 min before the end of the surgery,K),ketamine and fentanyl(infusion of 15 ?g?kg-1?min-1 ketamine from the skin incision until 20 min before the end of the surgery plus 3 bolus of 1 ?g/kg fentanyl,at 15-min intervals,FK).The cumulative morphine consumption,pain score,and adverse effects(nausea, vomiting,hallucination,dizziness and itching) were recorded at 1,3,6,12,24,48 h postoperatively.RESULTS: There were no significant differences in age,weight,duration of surgery and the post-operative sensory block time.The cumulative morphine consumption in group F was significantly higher than those in group C at 3,6,12 h postoperatively(P
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Objective To select the herba anti- human papillomavirus (HPV) active fraction from herba Arnebia. Methods The fractions from herba Arnebia. were separated with systematic solvents including petroleum arieal part of benzin, n- butanol , ethanol and distilled water, and their effects on HPV- DNA were evaluated by fluorescence quantitative polymerase chain reaction (FQ- PCR) technique. Results Only the water- extract of.this drug showed in- vitro inhibitory effect on HPV- DNA and its minimum effective concentration is 0.08g/mL. Conclusion Herba Arnebia. has in- vitro inhibitory effect on HPV- DNA and the active components exists in the water- extract.